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# Tommy Angelini <angelini@uiuc.edu>
# requesting 6 days, at minimum=6 days
# beamline 34ID, "FILAMENT TWIST IN F-ACTIN BUNDLES"
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# top:/home/www/beamtime-requests/req00340.txt
# UNICAT Member Beam Time Request  #340
# created Mon Dec 16 00:45:37 CST 2002
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beamline:
	34ID

collaboration:
	No

contact:
	angelini@uiuc.edu

days:
	6

description:
	A wide variety of cationic molecules can organize f-actin filaments into bundles.  The native helical symmetry of uncondensed f-actin filaments is not necessarily the preferred symmetry of bundled filaments, which will depend on the particular bundling mechanism and the kind of condensing agent used.  In previous experiments we have observed changes of filament twist in f-actin bundles condensed with multivalent ions.  Preliminary data suggests that a different twist state occurs when a globular cationic protein is used instead of multivalent ions to consense f-actin.  In order to explore the role of filament distortion in the process of bundle formation, we will carry out a series of small angle x-ray scattering (SAXS) experiments at sector 34-ID-C on systems of f-actin condensed with globular proteins such as lysozyme and specific actin-binding proteins such as alpha-actinin.  The area detector (Princeton Instruments CCD camera) and variable-length detector arm make it possible to collect all of the important f-actin helix diffraction features simultaneously.

equipment+required:
	

experiment:
	FILAMENT TWIST IN F-ACTIN BUNDLES

foreign+nationals:
	

hazards:
	None.

minimumdays:
	6

name:
	Tommy Angelini

new+request:
	on

nonmembers:
	

unacceptable+dates:
	March 3 through March 7, 2002.


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