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# Thomas Angelini <angelini@uiuc.edu>
# requesting 6 days, at minimum=3 days
# beamline 34ID, "Filament Twist in F-actin Bundles"
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# top:/home/www/beamtime-requests/req00378.txt
# UNICAT Member Beam Time Request  #378
# created Fri Mar 14 11:53:07 CST 2003
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beamline:
	34ID

collaboration:
	No

contact:
	angelini@uiuc.edu

days:
	6

description:
	The twist state of F-actin can modify specific binding sites and 
	influence the hierarchy of interactions in cytoskeletal regulation. 
	F-actin in the cytoskeleton is often organized into bundles, using a 
	wide variety of cationic molecules and actin-linking proteins. The 
	native helical symmetry of uncondensed f-actin filaments is not 
	necessarily the preferred symmetry of bundled filaments, which, as
	our preliminary experiments have shown, depend on the particular 
	bundling mechanism and the kind of condensing agent used. 
	
	In order to explore the role of filament distortion in the process 
	of bundle formation, we will carry out SAXS measurements on systems
	of bundled f-actin, using a variety of bundling agents including 
	multivalent cations, cationic globular proteins (lysozyme), and 
	actin specific binding proteins (alpha-actinin and scruin).  All
	samples will be prepared at home in hermetically sealed quartz 
	capillaries.  No sample preparation will be done at APS.  2-d
	diffraction patterns will be taken using the Princeton Instruments 
	CCD camera at sector 34 ID-C.

equipment+required:
	

experiment:
	Filament Twist in F-actin Bundles

foreign+nationals:
	

hazards:
	

minimumdays:
	3

name:
	Thomas Angelini

nonmembers:
	

unacceptable+dates:
	8/22/03 through 8/30/03 and 7/1/03 through 7/14/03.


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