#################################################### # Tommy Angelini # requesting 6 days, at minimum=3 days # beamline 34ID, "FILAMENT TWIST IN F-ACTIN BUNDLES" #################################################### # top:/home/www/beamtime-requests/req00475.txt # UNICAT Member Beam Time Request #475 # created Fri Aug 29 15:35:04 CDT 2003 #################################################### beamline: 34ID collaboration: No contact: angelini@uiuc.edu days: 6 description: A variety of cationic molecules and actin-linking proteins can organize f-actin filaments into bundles. The native helical symmetry of uncondensed f-actin filaments is not necessarily the preferred symmetry of bundled filaments, which will depend on the particular bundling mechanism and the kind of condensing agent used. In order to explore the role of filament distortion in the process of bundle formation, we have carried out a series of SAXS measurements at Sector 34 ID-C on systems of bundled f-actin, using several kinds of condensing agents, such as multivalent ions, globular cationic proteins, and specific actin linking proteins. Further SAXS studies will be carried out in unexplored ranges of concentration and temperature. equipment_required: experiment: FILAMENT TWIST IN F-ACTIN BUNDLES foreign_nationals: hazards: minimumdays: 3 name: Tommy Angelini nonmembers: unacceptable_dates: 11/23-11/30 z34ID_change_undulator: no z34ID_details: I do not want the undulator tapered. z34ID_parasitic: yes #REMOTE_HOST: biomat2.mrl.uiuc.edu #REMOTE_ADDR: 130.126.102.179 #CONTENT_LENGTH: 1100 #HTTP_REFERER: http://www.uni.aps.anl.gov/unireq.htm #HTTP_USER_AGENT: Mozilla/4.0 (compatible; MSIE 6.0; Windows 98)