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# Tommy Angelini <angelini@uiuc.edu>
# requesting 6 days, at minimum=6 days
# beamline 34ID-E, FILAMENT TWIST IN F-ACTIN BUNDLES
# instrument 34ID-E microbeam
####################################################
# top:/home/www/beamtime-requests/req00540.txt
# UNICAT Member Beam Time Request  #540
# created Fri Dec 12 16:29:38 CST 2003
####################################################

beamline:
	34ID-E

collaboration:
	No

contact:
	angelini@uiuc.edu

days:
	6

description:
	A variety of cationic molecules and actin-linking proteins can organize f-actin filaments into bundles. The native helical symmetry of uncondensed f-actin filaments is not necessarily the preferred symmetry of bundled filaments, which will depend on the particular bundling mechanism and the kind of condensing agent used. In order to explore the roles of filament twist and electrostatics in the process of bundle formation, we have carried out a series of SAXS measurements at Sector 34 ID-C on systems of bundled f-actin, using several kinds of condensing agents, such as multivalent ions, globular cationic proteins, and specific actin linking proteins. Further SAXS studies will be carried out on F-actin bundles stabilized by a variety of F-actin linking agents.  We have also developed micro-fluidic devices used for aligning samples of condensed biomolecules.  Tests on these micro-fluidic devices will also be performed.

equipment_required:
	

experiment:
	FILAMENT TWIST IN F-ACTIN BUNDLES

foreign_nationals:
	

hazards:
	No hazardous materials will be brought to the APS.

instrument:
	34ID-E microbeam

instrument_other:
	

minimumdays:
	6

name:
	Tommy Angelini

nonmembers:
	

unacceptable_dates:
	We specifically request the dates of Feb 12-18.  March 22-26 are bad due to American Physical Society meeting.

z34ID_change_undulator:
	no

z34ID_details:
	

z34ID_parasitic:
	yes


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