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# Thomas Angelini <angelini@uiuc.edu>
# requesting 6 days, at minimum=6 days
# beamline 34ID-C, Filament Twist and Linker Distribution in F-actin Bundles
# instrument 34ID-C coherent
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# top:/home/www/beamtime-requests/req00672.txt
# UNICAT Member Beam Time Request  #672
# created Wed Aug 25 12:21:19 CDT 2004
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beamline:
	34ID-C

collaboration:
	No

contact:
	angelini@uiuc.edu

days:
	6

description:
	A variety of cationic molecules and actin-linking proteins can organize f-actin filaments into bundles and networks. The native helical symmetry of uncondensed f-actin filaments is not necessarily the preferred symmetry of bundled filaments, which will depend on the particular bundling mechanism and the kind of condensing agent used. In order to explore the roles of filament twist and electrostatics in the process of bundle and network formation, we will carry out a series of SAXS measurements at Sector 34 ID-C on systems of bundled f-actin, using several kinds of condensing agents, such as multivalent ions, globular cationic proteins, and specific actin linking proteins such as alpha-actinin and fimbrin. Additionally, by carefully setting the q-resolution we will perform lineshape measurements on multivalent ion condensed f-actin networks. We have also developed micro-fluidic devices used for aligning samples of condensed biomolecules. Tests on these micro-fluidic devices will also be performed.

equipment_required:
	

experiment:
	Filament Twist and Linker Distribution in F-actin Bundles

foreign_nationals:
	

hazards:
	None

instrument:
	34ID-C coherent

instrument_other:
	

minimumdays:
	6

name:
	Thomas Angelini

nonmembers:
	

unacceptable_dates:
	Desired Dates: Oct 20-26.  Unacceptible dates: Sept 23-Oct 10.

z34ID_change_undulator:
	no

z34ID_details:
	

z34ID_parasitic:
	yes


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